Post-transcriptional modifications in tRNA stabilize its tertiary structure, introduce recognition determinants and antideterminants towards RNA-interacting macromolecules and fine-tune the decoding process at the level of both efficiency and fidelity. Moreover, it is becoming increasingly clear that many aspects of RNA metabolism and function are regulated through the dynamic introduction and removal of modifications (1,2). Dynamic tRNA modification regulates cellular response in response to environmental stress and toxicant exposures (3,4,5). Cellular regulation of sulfur-containing tRNAs was found to be tightly coupled and coregulated with translation, in particular upon an increase of temperature (6,7).
We have previously studied biochemically and structurally several sulfuration enzymes that target uridine at positions 34 or 54 in tRNAs (8-11). We have shown that they use a [4Fe-4S] cluster as a cofactor, which led us to propose a new sulfuration mechanism, in which the cluster binds and activates the sulfur atom of the substrate (8).
The structure of E. coli apo-MnmA, which sulfurates U34 in tRNAs, is known for long (12) but we want to obtain its structure with the [4Fe-4S] cluster (holo-MnmA) (9) to definitely establish that it is a [Fe-S]-dependent enzyme.
The project will consist in the crystallization of several holo-MnmA proteins from different bacteria. For some organisms, the highly purified protein is available in high quantity and the work will aim at reconstituting the cluster, purifying the holo-protein and trying to crystallize it. The structure will be solved by molecular replacement.
We want also to understand how sulfur is transferred from L-Cysteine to MnmA. The sulfur atom from free L-cysteine is first mobilized by a PLP-dependent L-cysteine desulfurase. Whereas some organisms like E. coli then use a TusABCDE relay system for sulfur transfer, in some species, the persulfide attached to a specific desulfurase is directly transferred to MnmA. We would like to crystallize such MnmA/desulfurase complexes to gain insight into the sulfur transfer step.