Projects in our team focus on mitochondrial dynamics with a specific interest on the mechanistic dissection of homotypic fusion of mitochondrial outer membranes. Our strategy is to decompose this process at the level of the cell on the whole mitochondrial network, at the level of the organelle on isolated mitochondria and at the level of the molecules on the molecular machinery promoting mitochondrial fusion. We thus analyze fusion of mitochondrial outer membranes at the scale of single molecules and expand its investigation toward its impact on the whole mitochondrial network. We combine the power of budding yeast genetics and cell biology with state-of-the-art imaging techniques, biochemistry and structural analysis of proteins by X-ray crystallography.